Part:BBa_K1492000:Design
Clickable Outer Membrane Protein x
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
To integrate a Non-natural amino acid in the sequence of CPX some modifications were made. Within the part in which originally random mutations were induced a codon was mutated into the amber stop codon TAG. With a specific tRNA it is possible to implement a Non-Natural amino acid at the place of the TAG codon. The TAG codon was introduced into the Peptide Library by Site-Directed Mutagenesis. A HA-tag was also added to the C-terminus of the protein using overhang primers to use in characterization. After the modifications the protein was renamed to Clickable Outer Membrane Protein X (COMPx).
Figure 2 Gene design of COMPx
Source
Circulairy Permutated OMPx. Rice, J. J.. "Bacterial display using circularly permuted outer membrane protein OmpX yields high affinity peptide ligands." Protein Science 15.4 (2006): 825-836. Print. http://www.addgene.org/23336/